A Review Of 2-FDCK HCl
The lab says fixation will induce the dyes to leak with the cells. I feel the belief is usually that fixation may perhaps hurt mobile membranes. I did find a paper which describes fixation using methanol or acetic acid, just after staining which has a DCFDA by-product. DCFDA is the reagent that stains ROS during the package ab113851, and I believe